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Annexin V antibody

Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Flow cytometry (FACS). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250
Anti-Annexin V antibody used in Western Blot (WB). GTX103250

Cat No. GTX103250

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , GL261 , C8D30 , NIH-3T3 , Raw 264.7 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949660

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

annexin A5 , ANX5 , ENX2 , HEL-S-7 , PP4 , RPRGL3

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Flow cytometry (FACS). GTX103250

GTX103250 FACS Image

Annexin V antibody (GTX103250) detects Annexin V protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: Annexin V antibody (GTX103250) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw 264.7 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin V antibody used in Western Blot (WB). GTX103250

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169