GeneTex
United States (US)

Annexin V antibody

GTX103250_41017_20180105_WB_KO_watermark_19011506_222.jpg
GTX103250_41017_WB_19011506_388.jpg
GTX103250_41017_WB_M_19011506_230.jpg
GTX103250_41017_WB_R_19011506_659.jpg
GTX103250_41017_20181207_FACS_19011519_113.jpg

Cat No. GTX103250

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , GL261 , C8D30 , NIH-3T3 , Raw 264.7 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Annexin A5,Anx5,Enx2,Hel-S-7,Pp4,Rprgl3,Anxa5

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103250_41017_20180105_WB_KO_watermark_19011506_222.jpg

GTX103250 WB Image

Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX103250_41017_WB_19011506_388.jpg

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg HepG2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103250_41017_WB_M_19011506_230.jpg

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg Raw 264.7 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103250_41017_WB_R_19011506_659.jpg

GTX103250 WB Image

Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103250_41017_20181207_FACS_19011519_113.jpg

GTX103250 FACS Image

Annexin V antibody (GTX103250) detects Annexin V protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: Annexin V antibody (GTX103250) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169