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Annexin XI antibody, N-term

Anti-Annexin XI antibody, N-term used in Western Blot (WB). GTX89851
Anti-Annexin XI antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89851

Cat. No. GTX89851

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Annexin XI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Placenta shows strong staining of Basement Membrane in trophoblasts.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89851-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence YPGYPPPPGGYPP-C, from the N Terminus of the protein sequence according to NP_001148.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A11 , ALS23 , ANX11 , CAP-50 , CAP50

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Annexin XI antibody, N-term used in Western Blot (WB). GTX89851

GTX89851 WB Image

WB analysis of HeLa (A) and Jurkat (B) lysates using GTX89851 Annexin XI antibody, N-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Annexin XI antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89851

GTX89851 IHC-P Image

IHC-P analysis of human placenta using GTX89851 Annexin XI antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399