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Annexin XI antibody

KO/KD
Anti-Annexin XI antibody used in Western Blot (WB). GTX114255

Cat. No. GTX114255

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Annexin XI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin XI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620672

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A11 , ALS23 , ANX11 , CAP-50 , CAP50

Cellular Localization

Cytoplasm , Nucleus , Melanosome

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin XI antibody used in Western Blot (WB). GTX114255

GTX114255 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
Annexin XI antibody
GTX114255 diluted at 1:1000

REFERENCE

Application Reference

Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8) : E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Annexin XI antibody Cat. No. GTX114255

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Annexin XI antibody used in Immunoprecipitation (IP). GTX114255
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Lane Description :
SM: 10% starting material
UB:10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 ul of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Annexin XI antibody used in Western Blot (WB). GTX114255
Date : Anonymous submitted on 01-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and ANXA11 KO cells
Amount used : 50μg
Primary Antibody : 1:1000, °C, Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169