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Apc10 antibody

Orthogonal
Anti-Apc10 antibody used in Western Blot (WB). GTX23629
Anti-Apc10 antibody used in Western Blot (WB). GTX23629
Anti-Apc10 antibody used in Western Blot (WB). GTX23629
Anti-Apc10 antibody used in Western Blot (WB). GTX23629

Cat. No. GTX23629

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA, IHC

Reactivity

 Human
Package
100 μg ($589)
See all Apc10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1500
IP 1:100
ELISA 1:15000-1:80000
IHC 1:250-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids near the amino terminus of human APC10.

Purification

Purified by antigen-affinity chromatography.
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anaphase promoting complex subunit 10 , APC10 , DOC1

Background

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Database

Research Area

DATA IMAGES

Anti-Apc10 antibody used in Western Blot (WB). GTX23629

GTX23629 WB Image

Western blotting. Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa

Anti-Apc10 antibody used in Western Blot (WB). GTX23629

GTX23629 WB Image

Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.

Anti-Apc10 antibody used in Western Blot (WB). GTX23629

GTX23629 WB Image

Orthogonal

WB analysis of various samples using GTX23629 Apc10 antibody.
Lane 1 : HeLa cell nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : 293T whole cell lysate
Loading : 20 μg
Dilution : 1:500

Anti-Apc10 antibody used in Western Blot (WB). GTX23629

GTX23629 WB Image

Orthogonal

WB analysis of HeLa whole cell lysate using GTX23629 Apc10 antibody.
Lane 1 : whole cell lysate
Lane 2 : Nuclear extract
Loading : 20 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589