Apelin Receptor antibody [HL4171]

Boiled and unboiled RPMI 2650 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Apelin Receptor antibody [HL4171] (GTX642985) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)