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Apolipoprotein A1 antibody [513]

Anti-Apolipoprotein A1 antibody [513] used in ELISA (ELISA). GTX15658
Anti-Apolipoprotein A1 antibody [513] used in IHC (Paraffin sections) (IHC-P). GTX15658
Anti-Apolipoprotein A1 antibody [513] used in ELISA (ELISA). GTX15658
Anti-Apolipoprotein A1 antibody [513] used in Western Blot (WB). GTX15658
Anti-Apolipoprotein A1 antibody [513] used in Western Blot (WB). GTX15658

Cat. No. GTX15658

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 513

Isotype

 IgG1

Application

 WB, IHC-P, IP, ELISA, RIA

Reactivity

 Human
Package
100 μg ($399)
See all Apolipoprotein A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:10-1:100
IP Assay dependent
ELISA 1μg/ml
RIA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M Potassium Phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human plasma Apo A-1

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein A1 , apo(a)

Cellular Localization

Secreted

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein A1 antibody [513] used in ELISA (ELISA). GTX15658

GTX15658 ELISA Image

Sandwich ELISA analysis of recombinant human Apo A-1 or recombinant mouse Apo A-1 protein ranging from 1.6-1000ng/ml using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).

Anti-Apolipoprotein A1 antibody [513] used in IHC (Paraffin sections) (IHC-P). GTX15658

GTX15658 IHC-P Image

IHC-P analysis of human liver tissue sections using GTX15658 Apolipoprotein A1 antibody [513].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : microwaved for 10 minutes in 10mM sodium citrate, pH 6.0
Dilution : 1:50

Anti-Apolipoprotein A1 antibody [513] used in ELISA (ELISA). GTX15658

GTX15658 ELISA Image

Sandwich ELISA analysis of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).

Anti-Apolipoprotein A1 antibody [513] used in Western Blot (WB). GTX15658

GTX15658 WB Image

WB analysis of various samples using GTX15658 Apolipoprotein A1 antibody [513].
Left Lane : 10ul of serum depleted of highly abundant proteins using the Top 2 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO.
Middle Lane : 10ul of serum depleted of highly abundant proteins using the Top 12 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO.
Right Lane : 50 ug of HepG2 whole cell lysate
Dilution : 1:1000

Anti-Apolipoprotein A1 antibody [513] used in Western Blot (WB). GTX15658

GTX15658 WB Image

WB analysis of the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein or a recombinant mouse Apo A-1-hIgG1Fc fusion protein using GTX15658 Apolipoprotein A1 antibody [513].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399