GeneTex
United States (US)

Apolipoprotein A1 antibody [6001]

GTX15660_38_ELISA_19011519_202.jpg
GTX15660_1029_IHC-P_19011519_517.jpg
GTX15660_37_ELISA_19011519_865.jpg
GTX15660_1509_WB_19011519_214.jpg

Cat No. GTX15660

Host Mouse
Clonality Monoclonal
Clone Name 6001
Isotype IgG2a
Application WB, IHC-P, IP, ELISA, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
IP Assay dependent
ELISA 1μg/ml
RIA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot.
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human plasma Apo A-1

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apolipoprotein A1,Apo(A),Apoa1

Cellular Localization

Secreted

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES
GTX15660_38_ELISA_19011519_202.jpg

GTX15660 ELISA Image

Sandwich ELISA analysis of recombinant human Apo A-1 or recombinant mouse Apo A-1 protein ranging from 1.6-1000ng/ml using GTX15660 Apolipoprotein A1 antibody [6001] (detection antibody) and Apo A-1 rabbit oligoclonal antibody (capture antibody).

GTX15660_1029_IHC-P_19011519_517.jpg

GTX15660 IHC-P Image

IHC-P analysis of human liver tissue sections using GTX15660 Apolipoprotein A1 antibody [6001].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : microwaved for 10 minutes in 10mM sodium citrate, pH 6.0
Dilution : 1:100

GTX15660_37_ELISA_19011519_865.jpg

GTX15660 ELISA Image

Sandwich ELISA analysis of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) using GTX15660 Apolipoprotein A1 antibody [6001] (detection antibody) and Apo A-1 rabbit oligoclonal antibody (capture antibody).

GTX15660_1509_WB_19011519_214.jpg

GTX15660 WB Image

WB analysis of the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein or a recombinant mouse Apo A-1-hIgG1Fc fusion protein using GTX15660 Apolipoprotein A1 antibody [6001].
Dilution : 1:1000

Package List Price ($)
$ 319