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Apolipoprotein A1 antibody [HDL110]

Anti-Apolipoprotein A1 antibody [HDL110] used in ELISA (ELISA). GTX02896

Cat. No. GTX02896

Host

Mouse

Clonality

Monoclonal

Clone Name

HDL 110

Isotype

IgG1

Application

WB, ELISA, Sandwich ELISA

Reactivity

Human, Monkey
Package
250 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02896, Detection: GTX02897-02.

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

This antibody is able to detect native apolipoprotein A1 (apoA1), purified or in the form of HDL.The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HDL

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APOA1,apo(a),apolipoprotein A1,ApoA1,Apolipoprotein A1

Cellular Localization

Secreted

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein A1 antibody [HDL110] used in ELISA (ELISA). GTX02896

GTX02896 ELISA Image

Sandwich ELISA analysis of human apoA1 protein using GTX02896 Apolipoprotein A1 antibody [HDL110] as coating antibody and GTX02897-02 Apolipoprotein A1 antibody [HDL44] (Biotin) as detecting antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399