APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02896, Detection: GTX02897-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native apolipoprotein A1 (apoA1), purified or in the form of HDL.The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human HDL
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
APOA1,apo(a),apolipoprotein A1,ApoA1,Apolipoprotein A1
Cellular Localization
Secreted
Background
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
![Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02](/templates/default/js/lightbox/images/loading.gif "Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02")
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GTX02897-02 ELISA Image
Sandwich ELISA analysis of human apoA1 protein using GTX02896 Apolipoprotein A1 antibody [HDL110] as coating antibody and GTX02897-02 Apolipoprotein A1 antibody [HDL44] (Biotin) as detecting antibody.
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REFERENCE
There are currently no references for Apolipoprotein A1 antibody [HDL44] (Biotin) (GTX02897-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Apolipoprotein A1 antibody [HDL44] (Biotin) (GTX02897-02). Be the first to share your experience with this product.
![Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02](/upload/website/prouct_img/small/GTX02897-02/GTX02897-02_20210507_ELISA_w_23053123_836.webp "Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02")
![Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02](/upload/website/prouct_img/normal/GTX02897-02/GTX02897-02_20210507_ELISA_w_23053123_836.webp "Anti-Apolipoprotein A1 antibody [HDL44] (Biotin) used in ELISA (ELISA). GTX02897-02")