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Apolipoprotein A1 antibody [HL1713]

Recombinant antibody
Anti-Apolipoprotein A1 antibody [HL1713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637312
Anti-Apolipoprotein A1 antibody [HL1713] used in ELISA (ELISA). GTX637312
Anti-Apolipoprotein A1 antibody [HL1713] used in Western Blot (WB). GTX637312
Anti-Apolipoprotein A1 antibody [HL1713] used in Western Blot (WB). GTX637312

Cat. No. GTX637312

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1713

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Apolipoprotein A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Apolipoprotein A1 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein A1 , apo(a)

Cellular Localization

Secreted

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein A1 antibody [HL1713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637312

GTX637312 ICC/IF Image

Apolipoprotein A1 antibody [HL1713] detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Apolipoprotein A1 antibody [HL1713] used in ELISA (ELISA). GTX637312

GTX637312 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length human Apo-A1 Protein, His Tag (134.23-2.1 nM). Coated protein was probed with Apolipoprotein A1 antibody [HL1713] (GTX637312) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-Apolipoprotein A1 antibody [HL1713] used in Western Blot (WB). GTX637312

GTX637312 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein A1 antibody [HL1713] used in Western Blot (WB). GTX637312

GTX637312 WB Image

Mouse plasma and Rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations