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Apolipoprotein A1 antibody [mHDL36] (Biotin)

Anti-Apolipoprotein A1 antibody [mHDL36] (Biotin) used in ELISA (ELISA). GTX02898-02

Cat. No. GTX02898-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 mHDL36

Isotype

 IgG2a

Application

 WB, ELISA, Sandwich ELISA

Reactivity

 Mouse
Package
250 μg ($399)
See all Apolipoprotein A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02899, Detection: GTX02898-02.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

This antibody is able to detect native mouse apolipoprotein A1 (apoA1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Purified mouse apoA1

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein A1 antibody [mHDL36] (Biotin) used in ELISA (ELISA). GTX02898-02

GTX02898-02 ELISA Image

Sandwich ELISA analysis of mouse apoA1 protein using GTX02899 Apolipoprotein A1 antibody [mHDL93] as coating antibody and GTX02898-02 Apolipoprotein A1 antibody [mHDL36] (Biotin) as detecting antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399