Apolipoprotein A1 antibody

Cat. No. GTX112692

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Apolipoprotein A1 products

View product citations for antibody GTX112692 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Apolipoprotein A1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179007

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein A1 , apo(a)

Cellular Localization

Secreted

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Database

Gene ID: 335 APOA1
UniProt: P02647 APOA1

Research Area

DATA IMAGES

GTX112692 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112692 WB Image

Mouse plasma and rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112692 ICC/IF Image

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112692 ICC/IF Image

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.