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Apolipoprotein A1 antibody

Cat. No. GTX27614

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Mouse
Package
100 μg ($399)
See all Apolipoprotein A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:250-1:500
IP 1:100
ELISA 1:5000-1:10000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apolipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apolipoproteins.

PROPERTIES

Form

Liquid

Buffer

125mM Sodium Borate, 75mM NaCl, 5mM EDTA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Apolipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein A-I , Alp-1 , Apoa-1 , Brp-14 , Ltw-1 , Lvtw-1 , Sep-1 , Sep-2 , Sep2 , apo-AI , apoA-I

Cellular Localization

Secreted

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Database

Research Area

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399