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Apolipoprotein B antibody [6G6]

Anti-Apolipoprotein B antibody [6G6] used in Western Blot (WB). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in ELISA (ELISA). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

Cat No. GTX60445

Host

Mouse

Clonality

Monoclonal

Clone Name

6G6

Isotype

IgG1

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ApoB (AA: 3900 - 4110)expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887967

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein B antibody [6G6] used in Western Blot (WB). GTX60445

GTX60445 WB Image

WB analysis of human ApoB (AA: 3900-4110) recombinant protein using GTX60445 Apolipoprotein B antibody [6G6].

Anti-Apolipoprotein B antibody [6G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60445

GTX60445 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Apolipoprotein B antibody [6G6] used in ELISA (ELISA). GTX60445

GTX60445 ELISA Image

ELISA analysis of antigen using GTX60445 Apolipoprotein B antibody [6G6].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

GTX60445 FACS Image

FACS analysis of serum using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Purple : negative control

Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

GTX60445 FACS Image

FACS analysis of PBMC using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Purple : negative control

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379