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Apolipoprotein B antibody [6G6]

Anti-Apolipoprotein B antibody [6G6] used in ELISA (ELISA). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Western Blot (WB). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445
Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

Cat. No. GTX60445

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($419)
See all Apolipoprotein B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ApoB (AA: 3900 - 4110)expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887967

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein B antibody [6G6] used in ELISA (ELISA). GTX60445

GTX60445 ELISA Image

ELISA analysis of antigen using GTX60445 Apolipoprotein B antibody [6G6].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Apolipoprotein B antibody [6G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60445

GTX60445 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Apolipoprotein B antibody [6G6] used in Western Blot (WB). GTX60445

GTX60445 WB Image

WB analysis of human ApoB (AA: 3900-4110) recombinant protein using GTX60445 Apolipoprotein B antibody [6G6].

Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

GTX60445 FACS Image

FACS analysis of serum using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Purple : negative control

Anti-Apolipoprotein B antibody [6G6] used in Flow cytometry (FACS). GTX60445

GTX60445 FACS Image

FACS analysis of PBMC using GTX60445 Apolipoprotein B antibody [6G6].
Green : Apolipoprotein B
Purple : negative control

REFERENCE

Application Reference

Li H et al. Open Med (Wars) 2023; 18 (1) : 20230790 Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activity.
Sarhan MA et al. Sci Rep 2017; 7 (1) : 2495 Glycogen synthase kinase 3β inhibitors prevent hepatitis C virus release/assembly through perturbation of lipid metabolism.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419