GeneTex
United States (US)

Apolipoprotein B antibody [F2C4]

GTX15664_1030_IHC-P_19011519_292.jpg
GTX15664_277_ICC-IF_19011519_255.jpg

Cat No. GTX15664

Host Mouse
Clonality Monoclonal
Clone Name F2C4
Isotype IgG2a
Application ICC/IF, IHC-P, IP, ELISA, IHC, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 1:20
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

516 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human serum LDL

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apolipoprotein B,Fldb,Ldlcq4,Apob-100,Apob-48,Apob

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15664_1030_IHC-P_19011519_292.jpg

GTX15664 IHC-P Image

IHC-P analysis of human liver tissue using GTX15664 Apolipoprotein B antibody [F2C4].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX15664_277_ICC-IF_19011519_255.jpg

GTX15664 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX15664 Apolipoprotein B antibody [F2C4]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Package List Price ($)
$ 319