GeneTex
United States (US)

Apolipoprotein B antibody, Internal

GTX88654_889_IHC-P_18121411_842.jpg
GTX88654_888_IHC-P_18121411_257.jpg
GTX88654_2202_WB_18121411_843.jpg

Cat No. GTX88654

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
See all Apolipoprotein B products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 2μg/ml
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88654-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apolipoprotein B,Fldb,Ldlcq4,Apob-100,Apob-48,Apob

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88654_889_IHC-P_18121411_842.jpg

GTX88654 IHC-P Image

IHC-P analysis of human small intestine using GTX88654 Apolipoprotein B antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml
GTX88654_888_IHC-P_18121411_257.jpg

GTX88654 IHC-P Image

IHC-P analysis of human liver using GTX88654 Apolipoprotein B antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml
GTX88654_2202_WB_18121411_843.jpg

GTX88654 WB Image

WB analysis of human serum lysates using GTX88654 Apolipoprotein B antibody, Internal. This antibody showed background below 150kDa in serum samples.
Dilution : 0.5µg/ml
Package List Price ($)
$ 329