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Apolipoprotein B antibody [LDL11] (Biotin)

Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02

Cat No. GTX02900-02

Host

Mouse

Clonality

Monoclonal

Clone Name

LDL 11

Isotype

IgG1

Application

WB, ELISA, Sandwich ELISA

Reactivity

Human, Monkey
Package
250 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02901, Detection: GTX02900-02.

Not tested in other applications.

Calculated MW

516 kDa. ( Note )

Product Note

This antibody is able to detect native apoB in the form of LDL.The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human LDL

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

APOB,FLDB,LDLCQ4,apoB100,apoB48,apolipoprotein B,Apolipoprotein B

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02

GTX02900-02 ELISA Image

Sandwich ELISA analysis of human apoB protein using GTX02901 Apolipoprotein B antibody [LDL20 + LDL17] as coating antibody and GTX02900-02 Apolipoprotein B antibody [LDL11] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 379