APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02901, Detection: GTX02900-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native apoB in the form of LDL.The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human LDL
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
APOB,FLDB,LDLCQ4,apoB100,apoB48,apolipoprotein B,Apolipoprotein B
Cellular Localization
Cytoplasm,Secreted
Background
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02](/templates/default/js/lightbox/images/loading.gif "Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02")
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GTX02900-02 ELISA Image
Sandwich ELISA analysis of human apoB protein using GTX02901 Apolipoprotein B antibody [LDL20 + LDL17] as coating antibody and GTX02900-02 Apolipoprotein B antibody [LDL11] (Biotin) as detecting antibody.
Substrate : pNPP
|
REFERENCE
There are currently no references for Apolipoprotein B antibody [LDL11] (Biotin) (GTX02900-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Apolipoprotein B antibody [LDL11] (Biotin) (GTX02900-02). Be the first to share your experience with this product.
![Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02](/upload/website/prouct_img/small/GTX02900-02/GTX02900-02_20210507_ELISA_w_23053123_459.webp "Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02")
![Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02](/upload/website/prouct_img/normal/GTX02900-02/GTX02900-02_20210507_ELISA_w_23053123_459.webp "Anti-Apolipoprotein B antibody [LDL11] (Biotin) used in ELISA (ELISA). GTX02900-02")