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Apolipoprotein B antibody

Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994
Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994
Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994
Anti-Apolipoprotein B antibody used in ELISA (ELISA). GTX135994

Cat No. GTX135994

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Apolipoprotein B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994

GTX135994 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Apolipoprotein B antibody (GTX135994) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994

GTX135994 WB Image

Mouse plasma (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Apolipoprotein B antibody (GTX135994) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein B antibody used in Western Blot (WB). GTX135994

GTX135994 WB Image

Human plasma (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Apolipoprotein B antibody (GTX135994) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein B antibody used in ELISA (ELISA). GTX135994

GTX135994 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant apolipoprotein B (7.27-0.11 nM). Coated protein was probed with Apolipoprotein B antibody (GTX135994) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329
$ 169