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Apolipoprotein B antibody

Cat No. GTX27616

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA, IHC

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:1000
IP 1:100
ELISA 1:2000 - 1:10000
IHC 1:50 - 1:500
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

Product Note

Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.  This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.  Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.  Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

PROPERTIES

Form

Liquid

Buffer

0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

apoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379