*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:500
Not tested in other applications.
Product Note
Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Mouse
Immunogen
Mouse low density lipoprotein (LDL).
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_424227
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Apolipoprotein B , Ai315052 , Apo B-100 , Apob-100 , Apob-48 , Apob
Cellular Localization
Secreted
Background
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]