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Apolipoprotein B antibody

Anti-Apolipoprotein B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64377
Anti-Apolipoprotein B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64377

Cat. No. GTX64377

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Apolipoprotein B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

516 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human APOB (NP_000375.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein B , FLDB , LDLCQ4 , apoB-100 , apoB-48

Cellular Localization

Cytoplasm,Secreted

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64377

GTX64377 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64377 Apolipoprotein B antibody.
Blue : DAPI
Dilution : 1:100

Anti-Apolipoprotein B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64377

GTX64377 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64377 Apolipoprotein B antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399