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Apolipoprotein CIII antibody

Anti-Apolipoprotein CIII antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129994
Anti-Apolipoprotein CIII antibody used in Western Blot (WB). GTX129994
Anti-Apolipoprotein CIII antibody used in Western Blot (WB). GTX129994

Cat No. GTX129994

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

human plasma

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Apolipoprotein CIII. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein C3 , APOCIII

Cellular Localization

Secreted

Background

This gene encodes a protein component of triglyceride (TG)-rich lipoproteins (TRLs) including very low density lipoproteins (VLDL), high density lipoproteins (HDL) and chylomicrons. The encoded protein plays a role in role in the metabolism of these TRLs through multiple modes. This protein has been shown to promote the secretion of VLDL1, inhibit lipoprotein lipase enzyme activity, and delay catabolism of TRL remnants. Mutations in this gene are associated with low plasma triglyceride levels and reduced risk of ischemic cardiovascular disease, and hyperalphalipoproteinemia, which is characterized by elevated levels of high density lipoprotein (HDL) and HDL cholesterol in human patients. This gene and other related genes comprise an apolipoprotein gene cluster on chromosome 11. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein CIII antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129994

GTX129994 ICC/IF Image

Apolipoprotein CIII antibody detects Apolipoprotein CIII protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein CIII protein stained by Apolipoprotein CIII antibody (GTX129994) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein CIII antibody used in Western Blot (WB). GTX129994

GTX129994 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Apolipoprotein CIII antibody (GTX129994) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein CIII antibody used in Western Blot (WB). GTX129994

GTX129994 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Apolipoprotein C3 antibody (GTX129994) diluted at 1:10000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169