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Apolipoprotein D antibody

Anti-Apolipoprotein D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101306
Anti-Apolipoprotein D antibody used in Western Blot (WB). GTX101306
Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306

Cat No. GTX101306

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apolipoprotein D

Cellular Localization

Secreted

Background

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Apolipoprotein D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101306

GTX101306 ICC/IF Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein D protein stained by Apolipoprotein D antibody (GTX101306) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein D antibody used in Western Blot (WB). GTX101306

GTX101306 WB Image

Apolipoprotein D antibody detects Apolipoprotein D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D antibody (GTX101306) diluted at 1:1000.

Anti-Apolipoprotein D antibody used in IHC (Paraffin sections) (IHC-P). GTX101306

GTX101306 IHC-P Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Apolipoprotein D antibody (GTX101306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169