GeneTex
United States (US)

Apolipoprotein D antibody

GTX101306_42151_20151030_IFA_19011506_760.jpg
GTX101306_41983_20150827_WB_19011506_751.jpg
GTX101306_42088_20150814_IHC-P_R_19011506_128.jpg

Cat No. GTX101306

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APOD antibody, apolipoprotein D antibody, apo-D antibody

Cellular Localization

Secreted

Background

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101306_42151_20151030_IFA_19011506_760.jpg

GTX101306 ICC/IF Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein D protein stained by Apolipoprotein D antibody (GTX101306) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101306_41983_20150827_WB_19011506_751.jpg

GTX101306 WB Image

Apolipoprotein D antibody detects Apolipoprotein D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D antibody (GTX101306) diluted at 1:1000.

GTX101306_42088_20150814_IHC-P_R_19011506_128.jpg

GTX101306 IHC-P Image

Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Apolipoprotein D antibody (GTX101306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169