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Apolipoprotein E antibody [1H4]

Anti-Apolipoprotein E antibody [1H4] used in Flow cytometry (FACS). GTX60446
Anti-Apolipoprotein E antibody [1H4] used in ELISA (ELISA). GTX60446
Anti-Apolipoprotein E antibody [1H4] used in Western Blot (WB). GTX60446
Anti-Apolipoprotein E antibody [1H4] used in IHC (Paraffin sections) (IHC-P). GTX60446

Cat. No. GTX60446

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ApoE expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887968

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody [1H4] used in Flow cytometry (FACS). GTX60446

GTX60446 FACS Image

FACS analysis of HepG2 cells using GTX60446 Apolipoprotein E antibody [1H4].
Green : Apolipoprotein E
Purple : negative control

Anti-Apolipoprotein E antibody [1H4] used in ELISA (ELISA). GTX60446

GTX60446 ELISA Image

ELISA analysis of antigen using GTX60446 Apolipoprotein E antibody [1H4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Apolipoprotein E antibody [1H4] used in Western Blot (WB). GTX60446

GTX60446 WB Image

WB analysis of HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate using GTX60446 Apolipoprotein E antibody [1H4].

Anti-Apolipoprotein E antibody [1H4] used in IHC (Paraffin sections) (IHC-P). GTX60446

GTX60446 IHC-P Image

IHC-P analysis of liver cancer tissue (left) and brain tissue (right) using GTX60446 Apolipoprotein E antibody [1H4].

REFERENCE

Application Reference

Sarhan MA et al. Sci Rep 2017; 7 (1) : 2495 Glycogen synthase kinase 3β inhibitors prevent hepatitis C virus release/assembly through perturbation of lipid metabolism.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409