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Apolipoprotein E antibody [C2C3], C-term

GTX100053_39233_CT_WB_19011506_328.jpg
GTX100053_39233_IFA_19011506_161.jpg
GTX100053_43006_20171220_ICC_IF_19011506_531.jpg
GTX100053_42963_20171027_WB_H_plasma_19011506_343.jpg
GTX100053_39233_IP_19011506_619.jpg
GTX100053_39233_WB_19011506_331.jpg
GTX100053_39233_IHC_19011506_522.jpg
GTX100053_42963_20180907_ELISA_19011506_173.jpg

Cat No. GTX100053

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2 , Conditional medium from differentiated human primary preadipocyte , human plasma

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AD2 antibody, LDLCQ5 antibody, LPG antibody, MGC1571 antibody, APOE antibody, apolipoprotein E antibody, apolipoprotein E3 antibody, apo-E antibody

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100053_39233_CT_WB_19011506_328.jpg

GTX100053 WB Image

Sample (2.5 µg of whole cell lysate)
A: Conditional medium from human primary preadipocyte
B: Conditional medium from differentiated human primary preadipocyte
12% SDS PAGE
GTX100053 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100053_39233_IFA_19011506_161.jpg

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100053_43006_20171220_ICC_IF_19011506_531.jpg

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100053_42963_20171027_WB_H_plasma_19011506_343.jpg

GTX100053 WB Image

Human plasma (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100053_39233_IP_19011506_619.jpg

GTX100053 IP Image

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term (GTX100053)
10% SDS-PAGE
The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1 : 1000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX100053_39233_WB_19011506_331.jpg

GTX100053 WB Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
12% SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100053_39233_IHC_19011506_522.jpg

GTX100053 IHC-P Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100053_42963_20180907_ELISA_19011506_173.jpg

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GTX100053 as capture antibody at concentration of 5 µg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169