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Apolipoprotein E antibody [C2C3], C-term

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

Cat No. GTX100053

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2 , Conditional medium from differentiated human primary preadipocyte , human plasma
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apolipoprotein E,AD2,APO-E,ApoE4,LDLCQ5,LPG

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Sample (2.5 µg of whole cell lysate)
A: Conditional medium from human primary preadipocyte
B: Conditional medium from differentiated human primary preadipocyte
12% SDS PAGE
GTX100053 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Human plasma (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053

GTX100053 IP Image

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term (GTX100053)
10% SDS-PAGE
The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1 : 1000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
12% SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GTX100053 as capture antibody at concentration of 5 µg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

GTX100053 IHC-P Image

Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169