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Apolipoprotein E antibody [C2C3], C-term

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

Cat No. GTX100053

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2 , Conditional medium from differentiated human primary preadipocyte , human plasma
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Sample (2.5 µg of whole cell lysate)
A: Conditional medium from human primary preadipocyte
B: Conditional medium from differentiated human primary preadipocyte
12% SDS PAGE
GTX100053 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Human plasma (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053

GTX100053 IP Image

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term (GTX100053)
10% SDS-PAGE
The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1 : 1000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
12% SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GTX100053 as capture antibody at concentration of 5 µg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

GTX100053 IHC-P Image

Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169