Apolipoprotein E antibody [C2C3], C-term

Name: Apolipoprotein E antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX100053
Price: 419.00 USD
Rating: 5 (2 reviews)

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053

Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

Cat. No. GTX100053

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μl ($419), 25 μl ($169)

See all Apolipoprotein E products

View product citations for antibody GTX100053 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IP	1:100-1:500
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635889 / GTX635891, Detection : GTX100053

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Conditional medium from differentiated human primary preadipocyte , HepG2 , human plasma

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949674

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Gene ID: 348 APOE
UniProt: P02649 APOE

Research Area

DATA IMAGES

GTX100053 WB Image

Sample (2.5 μg of whole cell lysate)
A: Conditional medium from human primary preadipocyte
B: Conditional medium from differentiated human primary preadipocyte
12% SDS PAGE
GTX100053 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT1627] (GTX635891) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX100053 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100053 IP Image

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term (GTX100053)
10% SDS-PAGE
The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:1000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GTX100053 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX100053 IHC-P Image

Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX100053 WB Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Adaku N et al. Cancer Res 2023; 83 (18) : 3013-3025 Apolipoprotein E2 Stimulates Protein Synthesis and Promotes Melanoma Progression and Metastasis.

Nilsa La Cunza et al. JCI Insight 2021; 6 (9) Mitochondria-dependent phase separation of disease-relevant proteins drives pathological features of age-related macular degeneration.

Application : IHC-Fr

Reactivity : Human

Lai W et al. Int J Nanomedicine 2020; 15 : 1481-1498 A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake.

Application : WB

Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.

Application : IHC-P

Reactivity : Human

Thaker K et al. Neurobiol Dis 2016; 93 : 64-77 Increased expression of ApoE and protection from amyloid-beta toxicity in transmitochondrial cybrids with haplogroup K mtDNA.

Application : ICC/IF

Reactivity : Human

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