APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
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Note :
Sandwich ELISA
Capture : GTX635889 / GTX635891, Detection : GTX100053
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909916
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG
Cellular Localization
Secreted
Background
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
![Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889](/templates/default/js/lightbox/images/loading.gif "Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889")
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GTX635889 WB Image
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635889 WB Image
Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635889 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E antibody [GT27711] (GTX635889) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.
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GTX635889 ELISA Image
Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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REFERENCE
There are currently no references for Apolipoprotein E antibody [GT27711] (GTX635889). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Apolipoprotein E antibody [GT27711] (GTX635889). Be the first to share your experience with this product.
![Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889](/upload/website/prouct_img/small/GTX635889/GTX635889_44195_20220225_WB_plasma_22030201_839.jpg "Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889](/upload/website/prouct_img/small/GTX635889/GTX635889_44195_20220225_WB_R_plasma_22030201_438.jpg "Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889](/upload/website/prouct_img/small/GTX635889/GTX635889_44195_20200122_ELISA_Indirect_21020419_890.jpg "Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889](/upload/website/prouct_img/small/GTX635889/GTX635889_44195_20200122_ELISA_PAIR_21020419_119.jpg "Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889](/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20220225_WB_plasma_22030201_839.jpg "Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889](/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20220225_WB_R_plasma_22030201_438.jpg "Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889](/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20200122_ELISA_Indirect_21020419_890.jpg "Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889")
![Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889](/upload/website/prouct_img/normal/GTX635889/GTX635889_44195_20200122_ELISA_PAIR_21020419_119.jpg "Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889")