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Apolipoprotein E antibody [GT27711]

KO/KD
Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889
Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889
Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889
Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889

Cat. No. GTX635889

Reference 1
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT27711

Isotype

 IgG2a

Application

 WB, IP, ELISA, Sandwich ELISA

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635889 / GTX635891, Detection : GTX100053
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909916

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889

GTX635889 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889

GTX635889 ELISA Image

Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889

GTX635889 WB Image

Rat plasma (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [GT27711] (GTX635889) diluted at 1:25000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [GT27711] used in ELISA (ELISA). GTX635889

GTX635889 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length human apolipoprotein E (400-6.25 ng/mL). Coated protein was probed with Apolipoprotein E antibody [GT27711] (GTX635889) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

Application Reference

Ayoubi R et al. F1000Res 2023; 12 : 810 Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.
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REVIEW

Apolipoprotein E antibody [GT27711] Cat. No. GTX635889

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Apolipoprotein E antibody [GT27711] used in Western Blot (WB). GTX635889
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and APOE KO culture media
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Apolipoprotein E antibody [GT27711] used in Immunoprecipitation (IP). GTX635889
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : Starved HAP1 culture media
Lane Description : SM=3% starting material UB=3% unbound fraction IP=immunoprecipitate HC=heavy chain
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. Starved HAP1 culture media were 0.6 ml aliquots at 1.5 mg/ml of protein were incubated with an antibody-bead conjugate for ~1 hr at 4ºC. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
Package List Price ($)
$ 399
$ 169