APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1/100-1/1,000 |
Assay dependent |
Note :
Neutralizing/Inhibition
Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).
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Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes an epitope within amino acids 140-160 of human Apolipoprotein E, a major component of very low-density lipoproteins (VLDLs).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified ApoHDL fraction.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG
Cellular Localization
Secreted
Background
The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX42746 ELISA Image
Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (GTX42746) as a capture reagent and biotinylated Goat anti Human Apolipoprotein E as a detection reagent. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown.
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GTX42746 ELISA Image
ELISA analysis of human apolipoprotein E protein using GTX42746 Apolipoprotein E antibody [WUE-4] (Low endotoxin, azide free).
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REFERENCE
There are currently no references for Apolipoprotein E antibody [WUE-4] (Low endotoxin, azide free) (GTX42746). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Apolipoprotein E antibody [WUE-4] (Low endotoxin, azide free) (GTX42746). Be the first to share your experience with this product.