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Apolipoprotein E antibody [WUE-4]

Anti-Apolipoprotein E antibody [WUE-4] used in ELISA (ELISA). GTX42745
Anti-Apolipoprotein E antibody [WUE-4] used in ELISA (ELISA). GTX42745

Cat. No. GTX42745

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 WUE-4

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($469)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA 1/100-1/1,000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Product Note

This antibody recognizes an epitope within amino acids 140-160 of human Apolipoprotein E, a major component of very low-density lipoproteins (VLDLs).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified ApoHDL fraction.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody [WUE-4] used in ELISA (ELISA). GTX42745

GTX42745 ELISA Image

Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (GTX42745) as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E as a detection reagent. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown.

Anti-Apolipoprotein E antibody [WUE-4] used in ELISA (ELISA). GTX42745

GTX42745 ELISA Image

ELISA analysis of human apolipoprotein E protein using GTX42745 Apolipoprotein E antibody [WUE-4].

REFERENCE

Clone Reference

Lasrich D et al. Exp Cell Res 2015; 337 (1):94-102 Apolipoprotein E promotes lipid accumulation and differentiation in human adipocytes.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469