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Apolipoprotein E antibody

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456
Anti-Apolipoprotein E antibody used in IHC (Paraffin sections) (IHC-P). GTX101456
Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456
Anti-Apolipoprotein E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101456

Cat No. GTX101456

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240464

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456

GTX101456 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody (GTX101456) diluted at 1:1000.

Anti-Apolipoprotein E antibody used in IHC (Paraffin sections) (IHC-P). GTX101456

GTX101456 IHC-P Image

Apolipoprotein E antibody detects Apolipoprotein E protein at secreted on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Apolipoprotein E antibody (GTX101456) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456

GTX101456 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody (GTX101456) diluted at 1:1000.

Anti-Apolipoprotein E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101456

GTX101456 ICC/IF Image

Apolipoprotein E antibody detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody (GTX101456) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169