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Apolipoprotein E antibody

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456
Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456
Anti-Apolipoprotein E antibody used in IHC (Paraffin sections) (IHC-P). GTX101456
Anti-Apolipoprotein E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101456

Cat. No. GTX101456

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , human plasma

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240464

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456

GTX101456 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody (GTX101456) diluted at 1:1000.

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX101456

GTX101456 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody (GTX101456) diluted at 1:1000.

Anti-Apolipoprotein E antibody used in IHC (Paraffin sections) (IHC-P). GTX101456

GTX101456 IHC-P Image

Apolipoprotein E antibody detects Apolipoprotein E protein at secreted on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Apolipoprotein E antibody (GTX101456) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Apolipoprotein E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101456

GTX101456 ICC/IF Image

Apolipoprotein E antibody detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody (GTX101456) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169