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Apolipoprotein E antibody

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX27620

Cat. No. GTX27620

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA, IHC

Reactivity

 Human, Rat
Package
100 μg ($399)
See all Apolipoprotein E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:100
ELISA 1:4000 - 1:8000
IHC 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Product Note

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.  This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins.  Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.  Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

PROPERTIES

Form

Liquid

Buffer

0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

Cellular Localization

Secreted

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein E antibody used in Western Blot (WB). GTX27620

GTX27620 WB Image

WB analysis lot of rat glial lysate (40 μg) using Apolipoprotein E antibody at a dilution of 1:1000.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399