GeneTex
United States (US)

Apolipoprotein E antibody

GTX27620_WB_18121410_142.jpg

Cat No. GTX27620

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IP, ELISA, IHC
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:100
ELISA 1:4000 - 1:8000
IHC 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Specificity/Sensitivity

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.  This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins.  Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.  Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
PROPERTIES

Form

Liquid

Buffer

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apolipoprotein E,Ad2,Apo-E,Apoe4,Ldlcq5,Lpg,Apoe

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Database

Research Area

DATA IMAGES
GTX27620_WB_18121410_142.jpg

GTX27620 WB Image

WB analysis lot of rat glial lysate (40 μg) using Apolipoprotein E antibody at a dilution of 1:1000.

Package List Price ($)
$ 289