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Apolipoprotein H antibody [N1C2]

Anti-Apolipoprotein H antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113627
Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627
Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627
Anti-Apolipoprotein H antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113627
Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627

Cat. No. GTX113627

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Apolipoprotein H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

human plasma , HepG2 sup.

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Apolipoprotein H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623318

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein H , B2G1 , B2GP1 , BG

Cellular Localization

Secreted

Background

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Apolipoprotein H antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113627

GTX113627 IHC-P Image

Apolipoprotein H antibody [N1C2] detects Apolipoprotein H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Apolipoprotein H stained by Apolipoprotein H antibody [N1C2] (GTX113627) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627

GTX113627 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein H antibody [N1C2] (GTX113627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627

GTX113627 WB Image

APOH antibody detects APOH protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APOH antibody (GTX113627) diluted at 1:1000.

Anti-Apolipoprotein H antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113627

GTX113627 IHC-P Image

Apolipoprotein H antibody [N1C2] detects secreted Apolipoprotein H protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Apolipoprotein H stained by Apolipoprotein H antibody [N1C2] (GTX113627) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein H antibody [N1C2] used in Western Blot (WB). GTX113627

GTX113627 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein H antibody [N1C2] (GTX113627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169