APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2
Predict Reactivity
Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Aprataxin Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949676
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aprataxin , AOA , AOA1 , AXA1 , EAOH , EOAHA , FHA-HIT
Cellular Localization
Nucleus , nucleoplasm , nucleolus
Background
This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109196 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Aprataxin(GTX109196) antibody at 1:500 dilution.
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GTX109196 IHC-P Image
Aprataxin antibody detects Aprataxin protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Aprataxin antibody (GTX109196) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109196 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Aprataxin (GTX109196) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109196 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX109196 diluted at 1:1000
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GTX109196 IHC-P Image
Aprataxin antibody detects Aprataxin protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Aprataxin antibody (GTX109196) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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