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Aquaporin 4 antibody

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151
Anti-Aquaporin 4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133151
Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151
Anti-Aquaporin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133151
Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX133151

Cat No. GTX133151

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Aquaporin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aquaporin 4 , WCH4

Cellular Localization

Membrane

Background

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Database

Research Area

DATA IMAGES

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151

GTX133151 IHC-P Image

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Aquaporin 4 antibody (GTX133151) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Aquaporin 4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133151

GTX133151 IHC-Fr Image

Aquaporin 4 antibody detects Aquaporin 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151

GTX133151 IHC-P Image

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Aquaporin 4 antibody (GTX133151) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Aquaporin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133151

GTX133151 ICC/IF Image

Aquaporin 4 antibody detects Aquaporin 4 protein at astrocyte cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX133151

GTX133151 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin 4 antibody (GTX133151) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169