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Aquaporin 4 antibody

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151
Anti-Aquaporin 4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133151
Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151
Anti-Aquaporin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133151
Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX133151

Cat No. GTX133151

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Aquaporin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aquaporin 4,WCH4

Cellular Localization

Membrane

Background

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

Database

Research Area

DATA IMAGES
Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151

GTX133151 IHC-P Image

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Aquaporin 4 antibody (GTX133151) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Aquaporin 4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133151

GTX133151 IHC-Fr Image

Aquaporin 4 antibody detects Aquaporin 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX133151

GTX133151 IHC-P Image

Aquaporin 4 antibody detects Aquaporin 4 protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Aquaporin 4 antibody (GTX133151) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Aquaporin 4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133151

GTX133151 ICC/IF Image

Aquaporin 4 antibody detects Aquaporin 4 protein at astrocyte cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aquaporin 4 protein stained by Aquaporin 4 antibody (GTX133151) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX133151

GTX133151 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin 4 antibody (GTX133151) diluted at 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169