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Aquaporin 4 antibody

Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX47920
Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX47920

Cat No. GTX47920

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Rat, Sheep, Bovine
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:100
IHC-P Assay dependent
IP 1:200
ELISA 1:10000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH 7.4-7.9, 30% glycerol, BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50-0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide taken within amino acid region 300-323 on rat Aquaporin 4 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aquaporin 4 , AQP-4 , Miwc , WCH4

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-Aquaporin 4 antibody used in Western Blot (WB). GTX47920

GTX47920 WB Image

WB analysis of Aquaporin 4 recombinant protein using GTX47920 Aquaporin 4 antibody.
Dilution : 1:500

Anti-Aquaporin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX47920

GTX47920 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX47920 Aquaporin 4 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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