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Arginase 1 antibody [ARG1/1125]

Anti-Arginase 1 antibody [ARG1/1125] used in IHC (Paraffin sections) (IHC-P). GTX34415
Anti-Arginase 1 antibody [ARG1/1125] used in Western Blot (WB). GTX34415

Cat No. GTX34415

Host Mouse
Clonality Monoclonal
Clone Name ARG1/1125
Isotype IgG3
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 2-4ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arginase 1,Arg1

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
Anti-Arginase 1 antibody [ARG1/1125] used in IHC (Paraffin sections) (IHC-P). GTX34415

GTX34415 IHC-P Image

Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1125).

Anti-Arginase 1 antibody [ARG1/1125] used in Western Blot (WB). GTX34415

GTX34415 WB Image

Western Blot Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).

Package List Price ($)
$ 329