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Arginase 1 antibody [GT5811]

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

Cat No. GTX634218

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5811

Isotype

IgG2a

Application

WB, IHC-P, FACS, ELISA, Sandwich ELISA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634218/GTX113131/GTX109242, Detection: GTX109242/GTX113131/GTX634218

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh-7 , ARG1-transfected 293T

Predict Reactivity

Rat, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888429

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218

GTX634218 IHC-P Image

Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218

GTX634218 FACS Image

Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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