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Arginase 1 antibody [GT5811]

Overexpression
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

Cat. No. GTX634218

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT5811

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Arginase 1 products
PRODUCT

Summary

Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634218 / GTX113131 / GTX109242, Detection : GTX109242 / GTX113131 / GTX634218
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh-7 , ARG1-transfected 293T

Product Note

This antibody is specific for human Arginase 1 protein, and it does not cross react with human Arginase 2 protein.

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218

GTX634218 IHC-P Image

Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218

GTX634218 FACS Image

Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

Application Reference

Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
Lee JD et al. Arch Toxicol 2020; 94 (3) : 887-909 Integration of transcriptomics, proteomics and metabolomics identifies biomarkers for pulmonary injury by polyhexamethylene guanidine phosphate (PHMG-p), a humidifier disinfectant, in rats.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations