Arginase 1 antibody [GT5811]

Cat No. GTX634218

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5811
Isotype	IgG2a
Application	WB, IHC-P, FACS, ELISA, Sandwich ELISA
Reactivity	Human, Mouse
Package	100 μl ($329), 25 μl ($169)

See all Arginase 1 products

PRODUCT

Summary

Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	1:50-1:200
ELISA	1:1000-1:10000
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX634218/GTX113131/GTX109242, Detection: GTX109242/GTX113131/GTX634218

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh-7 , ARG1-transfected 293T

Predict Reactivity

Rat, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888429

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Gene ID: 383 ARG1
UniProt: P05089 ARG1

Research Area

DATA IMAGES

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634218 IHC-P Image

Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634218 FACS Image

Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX634218 WB Image

Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Lee JD et al. Arch Toxicol 2020; 94 (3) : 887-909 Integration of transcriptomics, proteomics and metabolomics identifies biomarkers for pulmonary injury by polyhexamethylene guanidine phosphate (PHMG-p), a humidifier disinfectant, in rats.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW

There are currently no reviews for Arginase 1 antibody [GT5811] (GTX634218). Be the first to share your experience with this product.

Submit a Review

Application Reference

Lee JD et al. Arch Toxicol 2020; 94 (3):887-909 Integration of transcriptomics, proteomics and metabolomics identifies biomarkers for pulmonary injury by polyhexamethylene guanidine phosphate (PHMG-p), a humidifier disinfectant, in rats.

Application : WB

Reactivity : Human

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 329
25 μl	$ 169
Qty

Arginase 1 antibody [GT5811]

Cat No. GTX634218

Host

Clonality

Clone Name

Isotype

Application

Reactivity

Summary

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX634218 ELISA Image

GTX634218 ELISA Image

GTX634218 ELISA Image

GTX634218 ELISA Image

GTX634218 ELISA Image

GTX634218 WB Image

GTX634218 IHC-P Image

GTX634218 FACS Image

GTX634218 WB Image

REFERENCE

Application Reference

REVIEW

Citrate synthase antibody [GT1761]

PKM antibody

TOMM20 antibody

Product

Research

Support

Contact

Company

Follow Us

© 1998-2020 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.