PRODUCT
Summary
Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:50-1:200 |
1:1000-1:10000 |
Assay dependent |
Note :
Sandwich ELISA
Capture : GTX634218 / GTX113131 / GTX109242, Detection : GTX109242 / GTX113131 / GTX634218
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Not tested in other applications.
Calculated MW
Positive Control
Huh-7 , ARG1-transfected 293T
Product Note
This antibody is specific for human Arginase 1 protein, and it does not cross react with human Arginase 2 protein.
Predict Reactivity
Rabbit, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
3.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Arginase 1 recombinant protein.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888429
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
arginase 1
Cellular Localization
Cytoplasm
Background
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX634218 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634218 IHC-P Image
Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634218 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 FACS Image
Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX634218 ELISA Image
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 WB Image
Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX634218 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634218 ELISA Image
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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REFERENCE
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