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Arginase 1 antibody [GT5811]

Overexpression
GTX634218_42857_20171017_IHC-P_M_18121411_937.jpg
GTX634218_42857_20170525_WB_18121411_942.jpg
GTX634218_42857_20180831_ELISA_PAIR_B_18121411_927.jpg
GTX634218_42857_20181207_FACS_19011519_590.jpg
GTX634218_42857_20180831_ELISA_PAIR_B_2_18121411_708.jpg
GTX634218_42857_20180907_ELISA_PAIR_B_18121411_754.jpg
GTX634218_42857_20180831_ELISA_PAIR_B_3_18121411_743.jpg
GTX634218_42857_20180727_ELISA_PAIR_18121411_985.jpg
GTX634218_42857_20180727_ELISA_PAIR_2_18121411_725.jpg
GTX634218_42857_20170525_WB_B_18121411_124.jpg

Cat No. GTX634218

Host Mouse
Clonality Monoclonal
Clone Name GT5811
Isotype IgG2a
Application WB, IHC-P, FACS, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh-7 , ARG1-transfected 293T

Predict Reactivity

Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.26mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARG1 antibody , Arginase 1 antibody

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX634218_42857_20171017_IHC-P_M_18121411_937.jpg

GTX634218 IHC-P Image

Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634218_42857_20170525_WB_18121411_942.jpg

GTX634218 WB Image

Whole cell extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX634218_42857_20180831_ELISA_PAIR_B_18121411_927.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20181207_FACS_19011519_590.jpg

GTX634218 FACS Image

Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX634218_42857_20180831_ELISA_PAIR_B_2_18121411_708.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20180907_ELISA_PAIR_B_18121411_754.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX109242 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20180831_ELISA_PAIR_B_3_18121411_743.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX113131 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20180727_ELISA_PAIR_18121411_985.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20180727_ELISA_PAIR_2_18121411_725.jpg

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX634218_42857_20170525_WB_B_18121411_124.jpg

GTX634218 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169