Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
3.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Arginase 1 recombinant protein.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888429
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
arginase 1
Cellular Localization
Cytoplasm
Background
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 ELISA Image
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 ELISA Image
Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX634218 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634218 IHC-P Image
Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634218 FACS Image
Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX634218 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634218 WB Image
Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.