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Arginase 1 antibody [HL1891]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640
Anti-Arginase 1 antibody [HL1891] used in IHC (Paraffin sections) (IHC-P). GTX637640
Anti-Arginase 1 antibody [HL1891] used in IHC (Paraffin sections) (IHC-P). GTX637640
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640
Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

Cat. No. GTX637640

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1891

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Arginase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody is specific for human Arginase 1 protein, and it does not cross react with human Arginase 2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Arginase 1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

Rat tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [HL1891] used in IHC (Paraffin sections) (IHC-P). GTX637640

GTX637640 IHC-P Image

Arginase 1 antibody [HL1891] detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Arginase 1 stained by Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody [HL1891] used in IHC (Paraffin sections) (IHC-P). GTX637640

GTX637640 IHC-P Image

Arginase 1 antibody [HL1891] detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 stained by Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [HL1891] used in Western Blot (WB). GTX637640

GTX637640 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Currently in production
Custom Conjugations