PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Liver: A strong staining should be seen in hepatocytes. Spleen: A moderate t strong positivity should be seen in granulocytes.
Negative Control
Colon: Epithelial cells, smooth muscle and the vast majority of stroma cells should stain negative (few granulocytes may stain positive).
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human ARG1 protein fragment (aa300-400)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
arginase 1
Cellular Localization
Cytoplasm , Cytoplasmic granule
Background
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/templates/default/js/lightbox/images/loading.gif "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
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GTX04426 IHC-P Image
IHC-P analysis of human liver tissue section using GTX04426 Arginase 1 antibody [MSVA-511R] HistoMAX.
Strong nuclear and cytoplasmic arginase 1 expression in all hepatocytes in a normal liver.
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GTX04426 IHC-P Image
IHC-P analysis of human hepatocellular carcinoma tissue section using GTX04426 Arginase 1 antibody [MSVA-511R] HistoMAX.
Hepatocellular carcinoma showing strong nuclear and cytoplasmic arginase 1 positivity in all tumor cells.
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GTX04426 IHC-P Image
IHC-P analysis of human liver cholangiocellular carcinoma tissue section using GTX04426 Arginase 1 antibody [MSVA-511R] HistoMAX.
Complete absence of arginase 1 immunostaining in a cholangiocellular carcinoma of the liver.
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REFERENCE
There are currently no references for Arginase 1 antibody [MSVA-511R] HistoMAX (GTX04426). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Arginase 1 antibody [MSVA-511R] HistoMAX (GTX04426). Be the first to share your experience with this product.
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/small/GTX04426/GTX04426_20230728_IHC-P_4_23072722_512.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/small/GTX04426/GTX04426_20230728_IHC-P_134_23072722_985.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/small/GTX04426/GTX04426_20230728_IHC-P_264_23072723_195.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/normal/GTX04426/GTX04426_20230728_IHC-P_4_23072722_512.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/normal/GTX04426/GTX04426_20230728_IHC-P_134_23072722_985.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")
![Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426](/upload/website/prouct_img/normal/GTX04426/GTX04426_20230728_IHC-P_264_23072723_195.webp "Anti-Arginase 1 antibody [MSVA-511R] HistoMAX used in IHC (Paraffin sections) (IHC-P). GTX04426")