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Arginase 1 antibody

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242
Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242
Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242
Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

Cat No. GTX109242

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, sELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX109242 , Detection: GTX634218

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh7 , HepG2 , mouse liver , rat liver

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX109242 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242

GTX109242 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I (GTX109242) antibody at 1:200 dilution.

Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242

GTX109242 FACS Image

Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody (GTX109242) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242

GTX109242 IP Image

Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242).
Western blot analysis was performed using Arginase 1 antibody (GTX109242).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242

GTX109242 IHC-Fr Image

Arginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242

GTX109242 IHC-P Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Arginase 1 antibody Cat No. GTX109242

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 50μg
Blocking : 5% BSA, 26°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169