GeneTex
  • Country / Location Selection

United States (US)

Arginase 1 antibody

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242
Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242
Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242
Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

Cat. No. GTX109242

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634218 / GTX109242, Detection : GTX109242 / GTX634218

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242

GTX109242 IHC-P Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242

GTX109242 IP Image

Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242).
Western blot analysis was performed using Arginase 1 antibody (GTX109242) diluted at 1:600.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242

GTX109242 IHC-Fr Image

Arginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242

GTX109242 FACS Image

Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody (GTX109242) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242

GTX109242 ICC/IF Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal J Neuroinflammation in 2017.PMID: 28196545

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal PLoS One in 2014.PMID: 24658465

Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242

GTX109242 IHC-P Image

The data was published in the journal Oncotarget in 2016. PMID: 27637084

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal BMC Microbiol in 2017. PMID: 28835201

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28211494

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Physiol Rep in 2013. PMID: 24303100

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27637084

REFERENCE

REVIEW

Arginase 1 antibody Cat. No. GTX109242

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 14-Jul-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 10 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:6000, 4°C, 16Hr
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 50μg
Blocking : 5% BSA, 26°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Panebianco C et al. Cell Death Discov 2023; 9 (1):116
Application : IHC-P
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169