GeneTex
United States (US)

Arginase 1 antibody

KO/KD
GTX109242_42263_20160901_WB_shRNA_watermark_19011507_874.jpg
GTX109242_42263_20160728_WB_R_liver_19011507_125.jpg
GTX109242_IHC_19011507_608.jpg
GTX109242_42284_20160728_WB_M_liver_19011507_320.jpg
GTX109242_42998_20180907_ELISA_PAIR_B_19011507_235.jpg
GTX109242_IFA_19011507_708.jpg
GTX109242_42263_20160518_FACS_19011507_476.jpg
GTX109242_42998_20180831_ELISA_PAIR_B_19011507_587.jpg
GTX109242_42998_20180727_ELISA_PAIR_19011507_867.jpg
GTX109242_42284_20160219_IP_19011507_887.jpg
GTX109242_42914_20170720_WB_19011507_971.jpg
GTX109242_42914_20171017_IHC-P_M_19011507_719.jpg
GTX109242_42998_20181029_IHC-Fr_M_19011519_456.jpg

Cat No. GTX109242

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Huh7 , HepG2 , mouse liver , rat liver

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.06mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Arginase 1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARG1 antibody , arginase 1 antibody , type I arginase antibody , liver-type arginase antibody , arginase, liver antibody

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX109242_42263_20160901_WB_shRNA_watermark_19011507_874.jpg

GTX109242 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109242_42263_20160728_WB_R_liver_19011507_125.jpg

GTX109242 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109242_IHC_19011507_608.jpg

GTX109242 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I (GTX109242) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109242_42284_20160728_WB_M_liver_19011507_320.jpg

GTX109242 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000.

GTX109242_42998_20180907_ELISA_PAIR_B_19011507_235.jpg

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 µg/mL and GTX109242 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX109242_IFA_19011507_708.jpg

GTX109242 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I (GTX109242) antibody at 1:200 dilution.

GTX109242_42263_20160518_FACS_19011507_476.jpg

GTX109242 FACS Image

Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody (GTX109242) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX109242_42998_20180831_ELISA_PAIR_B_19011507_587.jpg

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX109242_42998_20180727_ELISA_PAIR_19011507_867.jpg

GTX109242 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 µg/mL and GTX634218 as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX109242_42284_20160219_IP_19011507_887.jpg

GTX109242 IP Image

Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242).
Western blot analysis was performed using Arginase 1 antibody (GTX109242).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109242_42914_20170720_WB_19011507_971.jpg

GTX109242 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109242_42914_20171017_IHC-P_M_19011507_719.jpg

GTX109242 IHC-P Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody (GTX109242) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109242_42998_20181029_IHC-Fr_M_19011519_456.jpg

GTX109242 IHC-Fr Image

Arginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW

Arginase 1 antibody Cat No. GTX109242

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 50μg
Blocking : 5% BSA, 26°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169