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Argonautes antibody [GT9003]

Anti-Argonautes antibody [GT9003] used in Immunoprecipitation (IP). GTX60370
Anti-Argonautes antibody [GT9003] used in Western Blot (WB). GTX60370
Anti-Argonautes antibody [GT9003] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60370
Anti-Argonautes antibody [GT9003] used in RNA Binding Protein Immunoprecipitation (RIP). GTX60370

Cat No. GTX60370

Host

Mouse

Clonality

Monoclonal

Clone Name

GT9003

Isotype

IgG1

Application

WB, ICC/IF, IP, IHC, RIP

Reactivity

Human, Mouse
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:500
IP 8 μg per IP
IHC 1:500
RIP Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

The antibody also recognizes other Ago family members although with the highest affinity for Ago2.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2).

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887962

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

argonaute RISC catalytic component 2 , CASC7 , EIF2C2 , LINC00980 , PPD , Q10

Cellular Localization

Nucleus

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Argonautes antibody [GT9003] used in Immunoprecipitation (IP). GTX60370

GTX60370 IP Image

IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded.
Antibody amount : 4 or 12μg.

Anti-Argonautes antibody [GT9003] used in Western Blot (WB). GTX60370

GTX60370 WB Image

WB analysis of whole cell extracts (40 μg) from HeLa cells using GTX60370 Argonautes antibody [GT9003].
Dilution : 1:500

Anti-Argonautes antibody [GT9003] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60370

GTX60370 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60370 Argonautes antibody [GT9003].
Red : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-Argonautes antibody [GT9003] used in RNA Binding Protein Immunoprecipitation (RIP). GTX60370

GTX60370 RIP Image

RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).

REFERENCE

REVIEW

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Package List Price ($)
$ 319