GeneTex
United States (US)

Arp2 antibody [N1C3]

GTX103311_40142_20150410_IFA_19011506_854.jpg
GTX103311_41087_IHC_M_19011506_874.jpg
GTX103311_40142_WB_M_19011506_603.jpg
GTX103311_40142_IHC_19011506_506.jpg
GTX103311_WB_19011506_718.jpg

Cat No. GTX103311

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Arp2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arp2 Actin Related Protein 2 Homolog,Arp2,Actr2

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection

Background

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103311_40142_20150410_IFA_19011506_854.jpg

GTX103311 ICC/IF Image

Arp2 antibody [N1C3] detects Arp2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Arp2 protein stained by Arp2 antibody [N1C3] (GTX103311) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX103311_41087_IHC_M_19011506_874.jpg

GTX103311 IHC-P Image

Arp2 antibody [N1C3] detects Arp2 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Arp2 antibody [N1C3] (GTX103311) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103311_40142_WB_M_19011506_603.jpg

GTX103311 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX103311 diluted at 1:1000

GTX103311_40142_IHC_19011506_506.jpg

GTX103311 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using Arp2(GTX103311) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103311_WB_19011506_718.jpg

GTX103311 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX103311 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169