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Arp2 antibody [N1C3]

Anti-Arp2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103311
Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311
Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311
Anti-Arp2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103311
Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311
Anti-Arp2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103311

Cat. No. GTX103311

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Arp2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Arp2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036160

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin related protein 2 , ARP2

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection

Background

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Arp2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103311

GTX103311 ICC/IF Image

Arp2 antibody [N1C3] detects Arp2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Arp2 protein stained by Arp2 antibody [N1C3] (GTX103311) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311

GTX103311 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Arp2 antibody [N1C3] (GTX103311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311

GTX103311 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arp2 antibody [N1C3] (GTX103311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arp2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103311

GTX103311 IHC-P Image

Arp2 antibody [N1C3] detects Arp2 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Arp2 antibody [N1C3] (GTX103311) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Arp2 antibody [N1C3] used in Western Blot (WB). GTX103311

GTX103311 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arp2 antibody [N1C3] (GTX103311) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arp2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103311

GTX103311 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human heart, using Arp2(GTX103311) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Saggioro M et al. Sci Rep 2023; 13 (1) : 9444 A perfusion-based three-dimensional cell culture system to model alveolar rhabdomyosarcoma pathological features.
Anne Pipathsouk et al. J Cell Biol 2021; 220 (8) The WAVE complex associates with sites of saddle membrane curvature.
Application : WB
Reactivity : Human
Graziano BR et al. PLoS Biol 2019; 17 (10) : e3000457 Cell confinement reveals a branched-actin independent circuit for neutrophil polarity.
Application : WB
Reactivity : Human
Butko MT et al. Proc Natl Acad Sci U S A 2013; 110 (8) : E726-35 In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.
Application : WB
Reactivity : Mouse
Chen HH et al. Mol Cell Biol 2012; 32 (12) : 2224-38 hnRNP Q regulates Cdc42-mediated neuronal morphogenesis.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169