GeneTex
United States (US)

Artemis antibody [N3C3]

GTX100128_40268_IHC_19011506_896.jpg
GTX100128_WB_19011506_466.jpg

Cat No. GTX100128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Artemis. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

A-SCID antibody, DCLREC1C antibody, FLJ11360 antibody, FLJ36438 antibody, RS-SCID antibody, SCIDA antibody, SNM1C antibody, hSNM1C antibody, DCLRE1C antibody, protein artemis antibody, SNM1-like protein antibody, SNM1 homolog C antibody, "severe combined immunodeficiency, type a (Athabascan) antibody", DNA cross-link repair 1C protein antibody, "DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae) antibody", PSO2 homolog antibody, DNA cross-link repair 1C antibody

Background

This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100128_40268_IHC_19011506_896.jpg

GTX100128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Artemis(GTX100128) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100128_WB_19011506_466.jpg

GTX100128 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
7.5% SDS PAGE
GTX100128 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169