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Artemis antibody [N3C3]

Anti-Artemis antibody [N3C3] used in Western Blot (WB). GTX100128
Anti-Artemis antibody [N3C3] used in Western Blot (WB). GTX100128
Anti-Artemis antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX100128

Cat No. GTX100128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Artemis. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNA cross-link repair 1C,A-SCID,DCLREC1C,RS-SCID,SCIDA,SNM1C

Background

This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Artemis antibody [N3C3] used in Western Blot (WB). GTX100128

GTX100128 WB Image

Artemis antibody detects Artemis protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
7.5 % SDS-PAGE
Artemis antibody (GTX100128) dilution: 1:1000

Anti-Artemis antibody [N3C3] used in Western Blot (WB). GTX100128

GTX100128 WB Image

Artemis antibody detects Artemis protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Artemis antibody (GTX100128) dilution: 1:1000

Anti-Artemis antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX100128

GTX100128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Artemis(GTX100128) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf