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Artemis antibody

Anti-Artemis antibody used in IHC (Paraffin sections) (IHC-P). GTX23834
Anti-Artemis antibody used in Western Blot (WB). GTX23834
Anti-Artemis antibody used in Western Blot (WB). GTX23834

Cat. No. GTX23834

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all Artemis products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:1000
ELISA 1:10000-1:20000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Product Note

This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) and in an hTERT line (not shown).

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding an internal region of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA cross-link repair 1C , A-SCID , DCLREC1C , RS-SCID , SCIDA , SNM1C

Cellular Localization

Nucleus

Background

Human Artemis, a new identified 78.4 KD, novel DNA double-strand break repair/v(d)j recombination protein. This gene is located at human chromosome 10. Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.

Database

Research Area

DATA IMAGES

Anti-Artemis antibody used in IHC (Paraffin sections) (IHC-P). GTX23834

GTX23834 IHC-P Image

GeneTex's Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded

Anti-Artemis antibody used in Western Blot (WB). GTX23834

GTX23834 WB Image

Western blotting. GeneTex's Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.

Anti-Artemis antibody used in Western Blot (WB). GTX23834

GTX23834 WB Image

WB analysis of various samples using GTX23834 Artemis antibody.
Lane 1 : AT7BI whole cell lysate
Lane 2 : M059K whole cell lysate
Lane 3 : M059J whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : CJ whole cell lysate
Dilution: 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589