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AspRS antibody [N1N3]

Anti-AspRS antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116291
Anti-AspRS antibody [N1N3] used in Western Blot (WB). GTX116291

Cat. No. GTX116291

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AspRS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AspRS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621254

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aspartyl-tRNA synthetase 2, mitochondrial , ASPRS , LBSL , MT-ASPRS

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AspRS antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116291

GTX116291 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AspRS(GTX116291) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AspRS antibody [N1N3] used in Western Blot (WB). GTX116291

GTX116291 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX116291 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169