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Asparaginase antibody (HRP)

Anti-Asparaginase antibody (HRP) used in Western Blot (WB). GTX40848
Anti-Asparaginase antibody (HRP) used in Western Blot (WB). GTX40848

Cat. No. GTX40848

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 E. coli
Package
1 mg ($399)
See all Asparaginase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ELISA 1:10000-1:40000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Asparaginase collected from E. coli.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_424293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity.E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Research Area

DATA IMAGES

Anti-Asparaginase antibody (HRP) used in Western Blot (WB). GTX40848

GTX40848 WB Image

Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody(GTX40848). Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.

Anti-Asparaginase antibody (HRP) used in Western Blot (WB). GTX40848

GTX40848 WB Image

WB analysis of E. coli asparaginase protein using GTX40848 Asparaginase antibody (HRP).
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Hu Y et al. Biomaterials 2021; 268 : 120606 An urchin-like helical polypeptide-asparaginase conjugate with mitigated immunogenicity.
Application : ELISA
Maria Morales-Gonzalez et al. Tropical Journal of Pharmaceutical Research 2018; 17 (11) : 2199-2206 Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia.
Application : WB
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Package List Price ($)
$ 399