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Asporin antibody, Internal

Anti-Asporin antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89589
Anti-Asporin antibody, Internal used in Western Blot (WB). GTX89589

Cat. No. GTX89589

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Asporin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1μg/ml
ICC/IF 10μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa cells.
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89589-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-IHENKVKKIQKDT, from the internal region of the protein sequence according to NP_060150.4.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

asporin , OS3 , PLAP-1 , PLAP1 , SLRR1C

Background

This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants.[provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-Asporin antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89589

GTX89589 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX89589 Asporin antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Asporin antibody, Internal used in Western Blot (WB). GTX89589

GTX89589 WB Image

WB analysis of human tonsil (A), human uterus (B), mouse skeletal muscle (C) rat skeletal muscle (D) and negative control human cerebellum (E) lysate using GTX89589 Asporin antibody, Internal.
Dilution : 0.1-1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399