GeneTex
United States (US)

Asporin antibody [N3C1], Internal

GTX104790_39694_20160303_WB_R_heart_19011506_166.jpg
GTX104790_39694_20160303_WB_M_liver_19011506_735.jpg
GTX104790_39694_IFA_19011506_664.jpg
GTX104790_39694_WB_19011506_722.jpg

Cat No. GTX104790

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , mouse liver , rat heart
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Asporin,Os3,Plap-1,Plap1,Slrr1C,Aspn

Background

ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM]

Database

DATA IMAGES
GTX104790_39694_20160303_WB_R_heart_19011506_166.jpg

GTX104790 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.

GTX104790_39694_20160303_WB_M_liver_19011506_735.jpg

GTX104790 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.

GTX104790_39694_IFA_19011506_664.jpg

GTX104790 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Asporin(GTX104790) antibody at 1:200 dilution.

GTX104790_39694_WB_19011506_722.jpg

GTX104790 WB Image

Asporin antibody [N3C1], Internal detects Asporin protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Asporin antibody [N3C1], Internal (GTX104790) dilution: 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169