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Ataxin 3 antibody [HL1909]

Recombinant antibody KO/KD
Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658
Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658

Cat. No. GTX637658

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1909

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Ataxin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Ataxin 3.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ataxin 3 , AT3 , ATX3 , JOS , MJD , MJD1 , SCA3

Background

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658

GTX637658 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658

GTX637658 IHC-P Image

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658

GTX637658 IHC-P Image

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658

GTX637658 IHC-P Image

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658

GTX637658 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658

GTX637658 ICC/IF Image

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658

GTX637658 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations