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Ataxin 3 antibody

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343
Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343
Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

Cat No. GTX101343

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , SK-N-SH , SK-N-AS , human Ataxin3-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ataxin 3 , AT3 , ATX3 , JOS , MJD , MJD1 , SCA3

Cellular Localization

Nucleus matrix

Background

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3.
10 % SDS-PAGE
Ataxin 3 antibody (GTX101343) dilution: 1:20000

Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343

GTX101343 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343

GTX101343 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody (GTX101343) diluted by 1:1000

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20414249, 27731380 and 27774050

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169