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Ataxin 3 antibody

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343
Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343
Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

Cat No. GTX101343

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , SK-N-SH , SK-N-AS , human Ataxin3-transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ataxin 3,AT3,ATX3,JOS,MJD,MJD1,SCA3

Cellular Localization

Nucleus matrix

Background

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3.
10 % SDS-PAGE
Ataxin 3 antibody (GTX101343) dilution: 1:20000

Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343

GTX101343 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Ataxin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101343

GTX101343 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX101343) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody (GTX101343) diluted by 1:1000

Anti-Ataxin 3 antibody used in Western Blot (WB). GTX101343

GTX101343 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20414249, 27731380 and 27774050

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169